In light sheet–based fluorescence microscopy (LSFM), optical sectioning in the excitation process minimizes fluorophore bleaching and phototoxic effects. Because biological specimens survive ...

Light-sheet fluorescence microscopy is a state-of-the art imaging technique for live-cell imaging. Unlike traditional imaging techniques such as laser scanning and spinning disk confocal ...

Light sheet microscopy with curved light sheets enables tiling-free imaging of an entire intact cleared mouse brain with lateral and axial spatial resolutions of 1.0 μm and 2.5 μm, respectively ...

Light-sheet microscopy is one of the most powerful method for imaging the development and function of whole living organisms. However, achieving high-resolution images with these microscopes ...

1. Development of a two-photon excitation light-sheet microscope which achieves low phototoxicity, an extended field of view, and high resolution for the observation of the growth of living ...

The first light-sheet microscope was created in 1903 by Siedentopf & Zsigmondy. 2 Their goal to analyze sub-resolution gold particles earned them the first-ever Nobel prize awarded for a ...

JENA, Germany & SEATTLE, May 06, 2025--ZEISS and Alpenglow Biosciences today announced a new partnership to jointly develop an inverted light-sheet microscope and bioinformatics pipeline tailored ...

In a light-sheet microscope, a laser light sheet illuminates the sample perpendicularly to the observation along a thin plane within the sample. Out-of-focus and scattered light from other planes - ...

Dr Bi-Chang Chen will discuss his research in developing cutting edge imaging tools for 3D live fluorescent microscopy, lattice light sheet microscopy. This will include an in-depth look at how Light ...

A research group led by Takashi Saito, of the Ehime University Graduate School of Medicine, developed a 2-photon excitation light-sheet fluorescence microscope which (1) lowers phototoxicity, (2 ...

Compared with confocal microscopy, SPIM takes images more gently and rapidly, which allows researchers to track biological processes in 3-D at higher resolution over longer time periods. There are ...

At the annual meeting of the American Society for Cell Biology (ASCB) in Philadelphia, PA, USA, from December 6 to 10, 2014, Leica Microsystems offers visitors a preview of its latest development: a ...